Read single-cell data into a Seurat object

Supports 10X Matrix, TXT, or RDS formats for raw count import.

Usage

read_sc_data(
  sc_path,
  project_name = NULL,
  method = c("10x", "txt", "rds"),
  gene.column = 1,
  min.cells = 3,
  min.features = 300,
  verbose = TRUE,
  ...
)

Arguments

sc_path

Character. Path to the data directory or file.

project_name

Character. Project name for Seurat object. Defaults to basename(sc_path) if NULL.

method

Character. Data format to import: "10X_matrix", "txt", or "rds". Default: "10X_matrix".

gene.column

Integer. Column index for gene names in 10X data. Default: 1.

min.cells

Integer. Minimum cells per feature for CreateSeuratObject. Default: 3.

min.features

Integer. Minimum features per cell for CreateSeuratObject. Default: 300.

verbose

Logical. Whether to print leo.log messages. Default: TRUE.

...

Additional arguments passed to CreateSeuratObject().

Value

A Seurat object of raw counts.